Science & Research

The Science Behind AYURMASTER

A research-informed approach to botanical formulation, quality selection and daily wellness product development.

Professor Dai Ayusawa in his laboratory
Prof. Dai Ayusawa, Professor Emeritus, Yokohama City University

Research Institution

Longevity Science Laboratory, Yokohama City University

Research Period

Over 30 years of cellular ageing and rejuvenation research

Published Journals

Journal of Integrative Medicine · Journal of Cosmetic Dermatology · Scientific Reports · Indian J Exp Biology

Japanese Patents Held

Patent Nos. 5935103 · 6977233 · 7193108 · 7452831

Herb Sourcing

Himalayan river-fed delta soil; HPLC lot-tested, ~20–30% acceptance rate

Chief Scientist

Prof. Dai Ayusawa, Professor Emeritus, Yokohama City University. 30+ years of published longevity and cellular ageing research.

Before You Read

This page explains the research background behind AYURMASTER's formulation philosophy. Research discussed includes peer-reviewed laboratory studies, cellular models, human monitoring studies and published papers, shared for educational purposes only.

They should not be interpreted as direct consumer claims. AYURMASTER products are not medicines and are not intended to diagnose, treat, cure or prevent any disease.

Published Measurements

What the Laboratory Recorded

Three findings from the Ayucelra®-HE research, with the model system stated for each.

Measured in a test tube

Antioxidant Activity

Methylene blue reduction assay, against two commercially available hydrogen waters.

Ayucelra-HE recorded a relative activity of 113, against 3 for one hydrogen water and no measurable activity for the other. That is more than 30 times the reducing power of the commercial samples tested.

Measured in human cells

Procollagen Gene Expression

Human facial dermal fibroblasts cultured with Amla fruit extract for 3 days; procollagen mRNA measured by Northern blot.

UV exposure cut procollagen expression to a third of its baseline. Adding Amla fruit extract raised it to 5–6 fold in the same UV-exposed cells.

Fold change relative to UV-exposed cells with no extract.

Measured in the bottle

Polyphenol Content

1,500–2,000 mg

Polyphenol per bottle of Ayucelra-HE, measured as catechin equivalent.

~200 mg

Polyphenol in a single glass of red wine, for comparison.

5–8 %

The share of ingested polyphenols absorbed through the gastric tract.

Source: Longevity Science Laboratory, Yokohama City University. These are laboratory measurements of the Ayucelra®-HE extract, not measured outcomes of finished AYURMASTER products in people. They are published here as research background and are not consumer claims.

From Research to Ritual

How a Formulation Is Developed

Six stages from traditional botanical record to a finished product.

Two cupped hands holding dried herbs, roots and bark

01

Study Traditional Use

Study botanicals from 5,000 years of Ayurvedic knowledge, with over 10,000 plant species documented by Ayurvedic physicians.

A researcher in a mask pipetting at a clean bench

02

Select Candidate Herbs

Screen from ~300 Ayurvedic herbs through cell-based assays to identify the most suitable combinations for each formulation.

A researcher in a mask working at an HPLC instrument

03

Analyse Quality (HPLC)

Every herb batch is tested by high-performance liquid chromatography. Approximately 70–80% of batches fail quality criteria and are rejected.

A researcher in a mask looking through an inverted microscope at a culture dish

04

Evaluate in Research Models

Test using human cell cultures, nematode (C. elegans) models, and human monitoring studies. Findings published in peer-reviewed journals.

Japanese patent certificate No. 5935103

05

Refine & Patent

Optimal herb combinations are refined and protected by Japanese patents (Patent Nos. 5935103, 6977233, 7193108, 7452831).

A worker in a cleanroom suit at a bottle filling and capping line

06

Made in Japan

Formulations are processed and finished at facilities in Japan; every product is labelled Made in Japan.

Herb Quality Inspection

Quality Testing, in Practice

HPLC chromatogram printout with many tall peaks across the run
Accepted lot: a full peak profile
HPLC chromatogram printout with a few peaks and a mostly flat baseline
Rejected lot: peaks missing

HPLC printouts from Ichiban Life's herb quality inspection records.

Research Background

The Unbalanced Growth Model of Cellular Senescence

Prof. Dai Ayusawa's landmark model (2005, Yokohama City University) demonstrates that under oxidative stress from UV, pollution and age, cells do not simply die. They grow excessively large and structurally unbalanced, entering irreversible growth arrest known as senescence. This model forms the scientific foundation of every Ayucelra® formulation.

Toggle below to visualise the difference between a balanced cell and a senescent cell.

The Ayucelra® Research Hypothesis

Research in Prof. Ayusawa's laboratory observed that specific Ayurvedic botanical combinations, later formulated as Ayucelra®, showed the ability to stimulate mitochondrial reactivation and support cellular proliferation in pre-senescent cell models. The hypothesis: certain polyphenol-rich herb synergies may help cells maintain balanced growth before irreversible arrest occurs.

This diagram is a simplified educational representation of laboratory research models. It does not represent a consumer claim or therapeutic effect.

AYUCELRA® BOTANICAL PROTECTIVE BARRIERNUCLEUSBALANCED CELL · CONTROLLED GROWTH
Cell Volume: NormalMetabolic State: ActiveProliferation: Controlled
Phase-contrast micrograph of compact colonies of small cells
Young cells
Phase-contrast micrograph of enlarged, flattened cells spread apart
Senescent cells
Phase-contrast micrographs of cultured cells from the laboratory.

01

Background Science

Cellular Ageing Research

The Longevity Science Laboratory at Yokohama City University has pursued cellular ageing and rejuvenation research for over 30 years. In 2005, the team published the 'Unbalanced Growth Model' of cellular senescence (Sumikawa et al., Biochem Biophys Res Commun), and in 2016 published its validation (Takauji et al., Scientific Reports). This work forms the scientific foundation behind AYURMASTER's formulation philosophy.

Research Insight

AYURMASTER's approach is shaped by research into how cells change over time and how Ayurvedic botanical formulations can be evaluated through modern cell science.

Peer-Reviewed References · PubMed

02

Health Care Drink

SOMA · Ayucelra®-HE

Ayucelra®-HE contains 40 Ayurvedic herbs with Amla fruit (Emblica officinalis) as the main botanical. Published research background includes: stimulation of epidermal keratinocyte proliferation by Emblica officinalis extract; and procollagen / proliferation activity of its active compound 1,6-digalloyl glucose in human keratinocytes and fibroblasts. Patent No. 5935103 covers the health drink formulation.

Research Insight

The Ayucelra®-HE research background informs SOMA's botanical selection, quality evaluation and product positioning as a daily wellness drink.

Peer-Reviewed References · PubMed

03

Women's Wellness Drink

MUYU-JU · Ayucelra®-Fem

Ayucelra®-Fem contains 15 Ayurvedic herbs with Saraca asoka as the main botanical, also including Asparagus racemosus (Shatavari), Emblica officinalis (Amla), Mangifera indica, Woodfordia fruticosa, Adhatoda vasica and Zingiber officinale. It was developed using the same laboratory cell-science methodology applied across the Ayucelra® range: botanical screening for cellular proliferation activity, antioxidant capacity and mitochondrial function in human cell models.

Research Insight

The MUYU-JU research background informs the selection of botanicals and the formulation philosophy for a women-focused daily wellness drink.

Research Evidence · Internal Study Data

  • —Antioxidant activity: reduced reactive-oxygen-species-induced DNA damage in human cell models.
  • —Cellular reactivation: restored mitochondrial activity in pre-senescent human cells.
  • —Human monitoring study (n=15, average age 48.7): self-assessed menopausal-comfort scores improved across all measured symptoms after one month; overall subject satisfaction 4.17 / 5.

MUYU-JU's evidence is internal research data generated by the Longevity Science Laboratory (Yokohama City University) and Ichiban Life Corporation, rather than indexed PubMed publications.

04

Hair Care Research

Scalp & Hair · Ayucelra®-SC

Ayucelra®-SC was developed after 10 years of research screening 300 Ayurvedic herbs, identifying 18 that act across the cell types involved in scalp and hair: keratinocytes, dermal fibroblasts, hair matrix cells, dermal papilla cells, melanocytes and sebaceous gland cells. Published work demonstrated that an extract of Plumbago zeylanica enhances the growth of hair follicle dermal papilla cells with down-regulation of 5α-reductase type II (J Cosmet Dermatol). The formulation and Scalp Cleansing shampoo are patented (Patent Nos. 6977233, 7193108).

Research Insight

The Ayucelra®-SC research background informs the botanical selection and formulation philosophy behind AYURMASTER's scalp and hair cosmetic range.

Peer-Reviewed References · PubMed

05

Skin Care Research

Skin Care · Ayucelra®-SK

Ayucelra®-SK was developed after 20 years of research, identifying 10 Ayurvedic herbs from 300 candidates for skin-related cell activity, including Emblica officinalis, Terminalia bellirica, Centella asiatica, Curcuma longa and Ellagic acid. Published research on Emblica officinalis and its active compound 1,6-digalloyl glucose demonstrated keratinocyte and fibroblast growth stimulation with upregulated periostin expression in human cell models.

Research Insight

The Ayucelra®-SK research background informs the botanical selection and formulation philosophy behind AYURMASTER's skin care cosmetic range.

Peer-Reviewed References · PubMed

Notice:The research information provided on this page is for educational and background purposes only. Laboratory studies, cell studies, animal studies and pre-clinical findings do not necessarily represent direct effects in humans. References to research do not mean that AYURMASTER products are medicines or that they can diagnose, treat, cure or prevent any disease.